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cd31 cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd31 cells
    (A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
    Cd31 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd31 cells - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Forward Programming Identifies Inducers of Blood-Brain Barrier Properties in Human Pluripotent Stem Cell-Derived Endothelial Cells"

    Article Title: Forward Programming Identifies Inducers of Blood-Brain Barrier Properties in Human Pluripotent Stem Cell-Derived Endothelial Cells

    Journal: bioRxiv

    doi: 10.64898/2026.02.02.699492

    (A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). CD31+ EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
    Figure Legend Snippet: (A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). CD31+ EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)

    Techniques Used: Over Expression, RNA Sequencing, Control, Sequencing, Expressing, Gene Expression



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    (A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). CD31+ EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)

    Journal: bioRxiv

    Article Title: Forward Programming Identifies Inducers of Blood-Brain Barrier Properties in Human Pluripotent Stem Cell-Derived Endothelial Cells

    doi: 10.64898/2026.02.02.699492

    Figure Lengend Snippet: (A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). CD31+ EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)

    Article Snippet: On day 5, EPCs were purified by magnetic sorting of CD31+ cells, using anti-CD31-biotin antibody (Miltenyi 130-110-667), anti-biotin microbeads (Miltenyi 130-097-046) and a QuadroMACS separator (Miltenyi 130-091-051).

    Techniques: Over Expression, RNA Sequencing, Control, Sequencing, Expressing, Gene Expression